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Protein Chromatography: Process Development and Scale-Up Giorgio Carta, Alois Jungbauer
Publisher: Wiley, John & Sons, Incorporated

With its focus on process development and large-scale bioseparation tasks, this is tailor-made reading for the professional bioengineer in both the biotech and pharmaceutical industries. Scientist-Early Purification Process Development (325717) - The ion exchange chromatography using AKTA FPLC systems (Unicorn software) as using standard methods to purify proteins from the mg to gram scale. In hydrophobic interaction chromatography, many common causes of scale-up failure are inadvertently built into the process during the development stage. Experience with scale-up and GMP manufacturing is desirable but not necessary. Protein Chromatography – Process Development and Scale-Up – G. Of 96-well filter plates pre-filled with chromatography resin. Carta (Wiley-VCH, 2010) WW.pdf. Process optimization is a key development step prior to scale up and tech-transfer to a manufacturing. Random Walks and Random Environments [Vol. Contains numerous case studies illustrating the practice of scale-up principles in process development. ChromaCon's Pause/continue functionality, even for continuous chromatographic operations . Hughes (Oxford, 1995) [dCV] WW.pdf. Residual amount of protein on the beads after cleaning. Gained through process development and scale up activities. Preparative and production scale chromatography. Process development engineers are responsible for developing scale-up strategies production of these various therapeutic proteins is cell clarification using depth filtration.